Unfamiliar with the ins and outs of Botox? That's not surprising. Although plenty of people are aware of Botox as a general concept, many don't quite understand how it works. Botox is a purified protein that, when injected, can reduce the activity of the muscles that cause frown lines to form over time. Within several days of receiving treatment, Botox can relax your muscles, resulting in smoother, younger-looking skin.